Macrophage galactose lectin is critical for Kupffer cells to clear aged platelets.

TitleMacrophage galactose lectin is critical for Kupffer cells to clear aged platelets.
Publication TypeJournal Article
Year of Publication2020
AuthorsDeppermann C, Kratofil RM, Peiseler M, David BA, Zindel J, Castanheira FVargas ES, van der Wal F, Carestia A, Jenne CN, Marth JD, Kubes P
JournalJ Exp Med
Volume217
Issue4
Date Published2020 Apr 06
ISSN1540-9538
Abstract

Every day, megakaryocytes produce billions of platelets that circulate for several days and eventually are cleared by the liver. The exact removal mechanism, however, remains unclear. Loss of sialic acid residues is thought to feature in the aging and clearance of platelets. Using state-of-the-art spinning disk intravital microscopy to delineate the different compartments and cells of the mouse liver, we observed rapid accumulation of desialylated platelets predominantly on Kupffer cells, with only a few on endothelial cells and none on hepatocytes. Kupffer cell depletion prevented the removal of aged platelets from circulation. Ashwell-Morell receptor (AMR) deficiency alone had little effect on platelet uptake. Macrophage galactose lectin (MGL) together with AMR mediated clearance of desialylated or cold-stored platelets by Kupffer cells. Effective clearance is critical, as mice with an aged platelet population displayed a bleeding phenotype. Our data provide evidence that the MGL of Kupffer cells plays a significant role in the removal of desialylated platelets through a collaboration with the AMR, thereby maintaining a healthy and functional platelet compartment.

DOI10.1084/jem.20190723
Alternate JournalJ. Exp. Med.
PubMed ID31978220
Grant ListP01 HL131474 / HL / NHLBI NIH HHS / United States
R01 DK048247 / DK / NIDDK NIH HHS / United States