Etchable plasmonic nanoparticle probes to image and quantify cellular internalization.

TitleEtchable plasmonic nanoparticle probes to image and quantify cellular internalization.
Publication TypeJournal Article
Year of Publication2014
AuthorsBraun GB, Friman T, Pang H-B, Pallaoro A, de Mendoza THurtado, Willmore A-MA, Kotamraju VRamana, Mann AP, She Z-G, Sugahara KN, Reich NO, Teesalu T, Ruoslahti E
JournalNat Mater
Volume13
Issue9
Pagination904-11
Date Published2014 Sep
ISSN1476-1122
KeywordsAnimals, Avidin, Biological Transport, Cell Line, Tumor, Cells, Female, Humans, Metal Nanoparticles, Mice, Molecular Imaging, Molecular Probes, Polyethylene Glycols, Silver
Abstract

There is considerable interest in using nanoparticles as labels or to deliver drugs and other bioactive compounds to cells in vitro and in vivo. Fluorescent imaging, commonly used to study internalization and subcellular localization of nanoparticles, does not allow unequivocal distinction between cell surface-bound and internalized particles, as there is no methodology to turn particles 'off'. We have developed a simple technique to rapidly remove silver nanoparticles outside living cells, leaving only the internalized pool for imaging or quantification. The silver nanoparticle (AgNP) etching is based on the sensitivity of Ag to a hexacyanoferrate-thiosulphate redox-based destain solution. In demonstration of the technique we present a class of multicoloured plasmonic nanoprobes comprising dye-labelled AgNPs that are exceptionally bright and photostable, carry peptides as model targeting ligands, can be etched rapidly and with minimal toxicity in mice, and that show tumour uptake in vivo.

DOI10.1038/nmat3982
Alternate JournalNat Mater
PubMed ID24907927
PubMed Central IDPMC4141013
Grant ListCA 030199 / CA / NCI NIH HHS / United States
P30 CA030199 / CA / NCI NIH HHS / United States
R01 CA152327 / CA / NCI NIH HHS / United States
R01 CA152327 / CA / NCI NIH HHS / United States
R01 CA167174 / CA / NCI NIH HHS / United States
R01 CA167174 / CA / NCI NIH HHS / United States
T32 CA121949 / CA / NCI NIH HHS / United States
T32 CA121949 / CA / NCI NIH HHS / United States