New p32/gC1qR Ligands for Targeted Tumor Drug Delivery.

TitleNew p32/gC1qR Ligands for Targeted Tumor Drug Delivery.
Publication TypeJournal Article
Year of Publication2016
AuthorsPaasonen L, Sharma S, Braun GB, Kotamraju VRamana, D Y Chung T, She Z-G, Sugahara KN, Yliperttula M, Wu B, Pellecchia M, Ruoslahti E, Teesalu T
JournalChembiochem
Volume17
Issue7
Pagination570-5
Date Published2016 Apr 1
ISSN1439-7633
Abstract

Cell surface p32, the target of LyP-1 homing peptide, is upregulated in tumors and atherosclerotic plaques and has been widely used as a receptor for systemic delivery of payloads. Here, we identified an improved LyP-1-mimicking peptide (TT1, CKRGARSTC). We used this peptide in a fluorescence polarization-based high-throughput screening of a 50,000-compound chemical library and identified a panel of compounds that bind p32 with low micromolar affinity. Among the hits identified in the screen, two compounds were shown to specifically bind to p32 in multiple assays. One of these compounds was chosen for an in vivo study. Nanoparticles surface-functionalized with this compound specifically adhered to surfaces coated with recombinant p32 and, when injected intravenously, homed to p32-expressing breast tumors in mice. This compound provides a lead for the development of p32-targeted affinity ligands that circumvent some of the limitations of peptide-based probes in guided drug delivery.

DOI10.1002/cbic.201500564
Alternate JournalChembiochem
PubMed ID26895508
Grant ListCA188883 / CA / NCI NIH HHS / United States
R01CA167174 / CA / NCI NIH HHS / United States
WT095077MA / / Wellcome Trust / United Kingdom